Jacqueline Sharon, Ph.D.
B.A./M.A. Queens College, New York
Ph.D. Columbia University, New York
A major area of interest in the laboratory involves the generation of polyclonal antibody libraries directed against human tumors (colorectal and breast) or against infectious agents (Neisseria gonorrhoeae and Cryptosporidium parvum). These libraries are collections of standardized antibodies directed to many different antigens on the target cells, and are obtained by cloning antibody genes and expressing antibody fragments on the surface of bacteriophage, generating Fab phage display libraries. Phage particles displaying antibody fragments specific for the target cell population are recovered and the genes encoding the desired antibodies transferred in mass to other vectors suitable for expression of whole antibodies. We are interested in studying the efficacy of such polyclonal antibody libraries at identifying or eliminating the target cells, both in vitro and in vivo, in preclinical experimental animal studies. The future goal is to employ polyclonal antibody libraries for the treatment and diagnosis of cancer and infectious diseases in humans.
Another project deals with the purification and characterization of a novel cytotoxic factor from mouse and human sera. This factor affects primarily tumor cells.