NGS Survey

Name*

Position*

What is your Primary Department or Program?*

If you are a PI, please list the other BU individuals who work on NGS projects with you.

If you are NOT a PI please list the PIs with whom you work on NGS projects.

What are your areas of expertise (select all that apply)

Computational/Statistical
Clinical
Wet-lab
Other

What other expertise do you have?

What species do you study?

Human
Mouse
Drosophila
Zebrafish
C. Elegans
Xenopus
Bacteria
Other

What specific bacteria species do you study?

What Other species do you study?

Which NGS technology (or technologies) are you currently using? (Check all that apply)

ChIP-Seq (chromatin IP - sequencing)
mRNA-Seq (exome, transciptome sequencing)
Small RNA analysis
Targeted resequencing
whole-genome resequencing
De novo whole-genome sequencing
Genome-wide methylation analysis
Other

What Other technologies do you use?

Which NGS platform do you utilize? (check all that apply)

Illumina Genome Analyzer (short read)
Applied Biosystems SOLiD (short read)
Roche (454) Gneome Sequencer FLX (long read)
Ion Torent PGM
Other

Which Other NGS platforms do you utilize?

What computational resources do you use for analysis of NGS data?

PCs within our own lab
Other computer resources within our own lab
LinGA
Bioinformatics Program cluster
Center for Scientific Computing and Visualization
Biomedical Engineering Cluster
Other

What Other computational resources do you use for analysis of NGS data?

What computational resource(s) do you use for storage of NGS data?

PCs within our own lab
Other computer resources within our own lab
LinGA
Bioinformatics Program cluster
Center for Scientific Computing and Visualization
Biomedical Engineering Cluster
Amazon Web Services
Other

What Other computational resource(s) do you use for storage of NGS data?

How do you backup your NGS data?

BUMC OIT Backup Service
Departmental backup
Portable drives
We don't
Other

What Other methods do you use to backup your NGS data?

For analyses of NGS data are you (check all that apply)

Utilizing the software provided by the company form which the instrument was purchased
Utilizing shareware software developed by academic researchers (e.g. Mosaik, Bowtie suite, etc)
Utilizing other commercially available software
Developing your own methodology and software
Other

What Other analyses of NGS data do you use?

For each NGS project that you are involved in please list the technology followed by the software used for processing, QC and analysis (e.g RNAseq: Bowtie, Cufflinks)

Which core or service are you presently using to generate your NGS data?

BU Illumina Core
Harvard Partners
Tufts
Broad
Other

What Other cores or services are you presently using to generate your NGS data?

What do you consider the biggest obstacle for taking advantage of NGS in your research?

Computational resources
Disk storage resources
Data management
Data analysis
Sample prep
Funding
Other

What Other obstacles prevent you from taking advantage of NGS in your research?

May we make the information provided above available to all individuals who signed up for the GSI NGS working group?*

Yes
No

May we make the information provided above available to all individuals at BU (Includes anyone with a Kerberos password, but will be Kerberos protected access)?

Yes
No

May we make the information provided above publicly available (listed on the GSI website with no restricted access?

Yes
No

Which of the following times are you available to meet on a regular basis for NGS working group meetings or seminars?*

Mondays, 12pm-1pm
Tuesday, 3pm-4pm
Wednesdays 10am-12pm
None of the above

If you are not currently on the NGS working group email list and would like to be added, please enter your email below.

Please provide any additional comments or information here.

Next Generation Sequencing Survey