Ph.D. Candidate, Molecular Medicine, BUSM
2006 B.S. Animal Science/Pre-Veterinary Medicice , University of New Hampshire
TGFβ signaling and Smad proteins in breast and colon cancer
Elucidating the effect of perturbing the TGFβ signaling pathway during different stages of breast tumorigenesis using a cell line model system. Knockdown of Smad2 using shRNA is accomplished with a lentiviral expression system. Analysis includes, PCR, reverse-transcription PCR, real-time PCR,
methylation-specific PCR, Western Blotting and chromatin immunoprecipitation.
Examine the effect of cyclic nucleotide phosphodiesterase inhibitors on lymphoma cells obtained from an Eµ-Brd2 transgenic mouse model for non-Hodgkin’s lymphoma
Propagated lymphomas in recipient mice, isolated malignant lymphocytes and
treated cells with various PDE inhibitors (rolipram, IBMX, cilostamide,
dipyridamole, etc.). Analyzed apoptosis by flow cytometry. In a separate
project, followed tumor progression and aberrant blow flow in mice using
Analysis of the klotho promoter region
Created various truncated reporter constructs using site-directed mutagenesis to be used in a luciferase assay to determine the necessary regulatory elements in the promoter region. Additionally, used promoter trapping chromatography and western blotting to identify Sp1 as a transcription factor that binds specifically to the -300 bp region upstream of the transcription start site in vitro.
Investigation of feeding anticipatory activity in offshore Atlantic cod
Anticipatory activity was investigated in a novel manner using underwater cameras and analyzed using MotionApp software to detect relative pixel change in order to evaluate feeding and pre-feeding behavior.
Gao, F., J. F. Ponte, P. Papageorgis, M. Levy, S. Ozturk, A. W. Lambert H. Pan, D. Chinnappan, B. Sullivan H. Abdolmaleky and S. Thiagalingam. 2009. hBub1 is a negative regulator of p53 mediated cell death upon activation of spindle assembly checkpoint. Manuscript submitted.
P. Papagerorgis, A.W. Lambert, S. Ozturk, H. Abdolmalcky and S. Thiagalingam. 2008. Smad signaling mediates epigenetic regulation of breast cancer progression. Evans Department of Medicine Research Days Abstract.
Papageorgis, P., A. W. Lambert, S. Ozturk, F. Gao, H. Abdolmaleky and S. Thiagalingam. 2008. Smad signaling mediates epigenetic regulation of EMT during breast cancer progression. Manuscript in preparation.