Plasmids
Available Plasmids
- Top10 pFP12-1870, resistance: chlorR
- Top10 pFP12-1870-mc06 (nrrF complementation plasmid), resistance: chlor R
- Top10 pSL-nrrF (derivative of pSL-Fur-C1 with nrrF gene), resistance: eryR
- Top10 -TOPO, resistance: kanR
- Top10 pSLFurE (pSL-FurC1 plasmid), resistance: eryR, ampR
- BL21-A1 pGex-4T2-Hfq (Hfq overexpression plasmid), resistance: ampR .5mM IPTG + 20% arabinose to induce
- BL21-A1 wild-type
- Top10 pSL-KA-Fur (pSLFurC1 plasmid with kanamycin cassete), resistance: kanR, ampR
- BL21-DE3 pGex-4T2 (GST overexpression plasmid), resistance: ampR .5mM IPTG to induce
- Top10 pSL-KA-Fur-HfqL (contains Hfq left flanking region for mutagenesis vector), resistance: kanR, ampR
- BL21-DE3 pGex-4T2-Hfq (GST-Hfq overexpression plasmid), resistance: ampR .5mM IPTG to induce
- Top10 pSL-KA-Hfq-LR (contains both Hfq flanking regions for mutagenesis vector), resistance: kanR, ampR
- RMP-lacZ (E.coli Top10 pBlue Topo comp cells + MC58 RMP Promoter-lacZ, resistance: ampR
- Fur-lacZ (E.coli pBlue + Topo comp cells + MC58 fur promoter-lacZ)
- nspa-lacZ (E.coli pBlue +Topo comp cells + MC58 nspa promoter-lacZ)
- sodB-lacZ (E.coli pBlue + topo comp cells +mc58 sodB promoter-lacZ)
- pgen, fkob:kn resistance: ampCplasmid kanR
- E. coli top 10 pLES95 (same as pLES94 except the left-flanking region is cloned from MC58 DNA not GC DNA), resistance: ampR, chlorR
- E. coli top 10 pLES94 (truncated lacZ gene), resistance: ampR, chlorR
- E. coli top 10 pLES94-sdhC, resistance: ampR, chlor
- E. coli top 10 pLES94-sodB, resistance: ampR, chlorR
- E. coli top 10 pLES94-mut1 (sdhC mutated promoter), resistance: ampR, chlorR
- Top10 pVEFasL , resistance: ampR
- Top10 pTLR2-YFP-polyA (From doug Gollenboch’s lab), resistance: ampR
- Top10 pSL-K-NrrF-C1 (NrrF complementation plasmid derviced from pSLFur-C1), resistance: kanR
- ples94 in top10
- pscfur in top 10 cells
- pgen empty vector
