Plasmids


Available Plasmids

  • Top10 pFP12-1870,   resistance: chlorR
  • Top10 pFP12-1870-mc06 (nrrF complementation plasmid), resistance: chlor R
  • Top10 pSL-nrrF (derivative of pSL-Fur-C1 with nrrF gene), resistance: eryR
  • Top10 -TOPO, resistance: kanR
  • Top10 pSLFurE (pSL-FurC1 plasmid), resistance: eryR, ampR
  • BL21-A1 pGex-4T2-Hfq (Hfq overexpression plasmid), resistance: ampR .5mM IPTG + 20% arabinose to induce
  • BL21-A1 wild-type
  • Top10 pSL-KA-Fur (pSLFurC1 plasmid with kanamycin cassete), resistance: kanR, ampR
  • BL21-DE3 pGex-4T2 (GST overexpression plasmid), resistance: ampR .5mM IPTG to induce
  • Top10 pSL-KA-Fur-HfqL (contains Hfq left flanking region for mutagenesis vector), resistance: kanR, ampR
  • BL21-DE3 pGex-4T2-Hfq (GST-Hfq overexpression plasmid), resistance: ampR .5mM IPTG to induce
  • Top10 pSL-KA-Hfq-LR (contains both Hfq flanking regions for mutagenesis vector), resistance: kanR, ampR
  • RMP-lacZ (E.coli Top10 pBlue Topo comp cells + MC58 RMP Promoter-lacZ, resistance: ampR
  • Fur-lacZ (E.coli pBlue + Topo comp cells + MC58 fur promoter-lacZ)
  • nspa-lacZ (E.coli pBlue +Topo comp cells + MC58 nspa promoter-lacZ) 
  • sodB-lacZ (E.coli pBlue + topo comp cells +mc58 sodB promoter-lacZ)
  • pgen, fkob:kn resistance: ampCplasmid kanR
  • E. coli top 10 pLES95 (same as pLES94 except the left-flanking region is cloned from MC58 DNA not GC DNA), resistance: ampR, chlorR
  • E. coli top 10 pLES94 (truncated lacZ gene), resistance: ampR, chlorR
  • E. coli top 10 pLES94-sdhC, resistance: ampR, chlor
  • E. coli top 10 pLES94-sodB, resistance: ampR, chlorR
  • E. coli top 10 pLES94-mut1 (sdhC mutated promoter), resistance: ampR, chlorR
  • Top10 pVEFasL , resistance: ampR
  • Top10 pTLR2-YFP-polyA (From doug Gollenboch’s lab), resistance: ampR
  • Top10 pSL-K-NrrF-C1 (NrrF complementation plasmid derviced from pSLFur-C1), resistance: kanR
  • ples94 in top10
  • pscfur in top 10 cells
  • pgen empty vector
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September 25, 2009
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of BU School of Medicine