Emergency BU Alert BU Medical Campus OPEN Jan. 28, 2015 Boston University Medical Campus will be open Wednesday, Jan. 28, 2015. BUSM classes will be held as scheduled. Staff should check with their managers regarding work schedules. Medical, PA and GMS students who are assigned to inpatient services or clinics are expected to be present, if possible. Students who are assigned to outpatient services should check with their course director or the policy at the clinical site. GMS classes are canceled. Staff should check with their manager regarding their work schedules. The Henry M. Goldman School of Dental Medicine will follow normal school hours. All Patient Treatment Centers will be open for patient care and all classes will be held as scheduled. BU School of Public Health classes are canceled; SPH non-essential staff may telecommute. Employees who are part of the BUMC parking program should park in your assigned lot or garage. The Boston parking ban is still in effect. For updated information, please call the weather/emergency hotline at 617-638-6886 or visit the BU Emergency Communications website at http://www.bu.edu/ehs/comm/

Plasmids


Available Plasmids

  • Top10 pFP12-1870,   resistance: chlorR
  • Top10 pFP12-1870-mc06 (nrrF complementation plasmid), resistance: chlor R
  • Top10 pSL-nrrF (derivative of pSL-Fur-C1 with nrrF gene), resistance: eryR
  • Top10 -TOPO, resistance: kanR
  • Top10 pSLFurE (pSL-FurC1 plasmid), resistance: eryR, ampR
  • BL21-A1 pGex-4T2-Hfq (Hfq overexpression plasmid), resistance: ampR .5mM IPTG + 20% arabinose to induce
  • BL21-A1 wild-type
  • Top10 pSL-KA-Fur (pSLFurC1 plasmid with kanamycin cassete), resistance: kanR, ampR
  • BL21-DE3 pGex-4T2 (GST overexpression plasmid), resistance: ampR .5mM IPTG to induce
  • Top10 pSL-KA-Fur-HfqL (contains Hfq left flanking region for mutagenesis vector), resistance: kanR, ampR
  • BL21-DE3 pGex-4T2-Hfq (GST-Hfq overexpression plasmid), resistance: ampR .5mM IPTG to induce
  • Top10 pSL-KA-Hfq-LR (contains both Hfq flanking regions for mutagenesis vector), resistance: kanR, ampR
  • RMP-lacZ (E.coli Top10 pBlue Topo comp cells + MC58 RMP Promoter-lacZ, resistance: ampR
  • Fur-lacZ (E.coli pBlue + Topo comp cells + MC58 fur promoter-lacZ)
  • nspa-lacZ (E.coli pBlue +Topo comp cells + MC58 nspa promoter-lacZ) 
  • sodB-lacZ (E.coli pBlue + topo comp cells +mc58 sodB promoter-lacZ)
  • pgen, fkob:kn resistance: ampCplasmid kanR
  • E. coli top 10 pLES95 (same as pLES94 except the left-flanking region is cloned from MC58 DNA not GC DNA), resistance: ampR, chlorR
  • E. coli top 10 pLES94 (truncated lacZ gene), resistance: ampR, chlorR
  • E. coli top 10 pLES94-sdhC, resistance: ampR, chlor
  • E. coli top 10 pLES94-sodB, resistance: ampR, chlorR
  • E. coli top 10 pLES94-mut1 (sdhC mutated promoter), resistance: ampR, chlorR
  • Top10 pVEFasL , resistance: ampR
  • Top10 pTLR2-YFP-polyA (From doug Gollenboch’s lab), resistance: ampR
  • Top10 pSL-K-NrrF-C1 (NrrF complementation plasmid derviced from pSLFur-C1), resistance: kanR
  • ples94 in top10
  • pscfur in top 10 cells
  • pgen empty vector
Contact|Directory|BUMC
September 25, 2009
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of BU School of Medicine