Performance of wounding experiments to test investigational agents in mouse full-thickness tail wounds

This model requires more explanation of its details. Figure 3a shows the basic formation of the tail wound and the histological analysis. Figure 3b shows results obtained in db/db mice (lower mouse photo) and its control littermate (upper photo).

Fig 3a

Fig.  3a. Left panel shows full-thickness tail wound down to fascia (arrow). Right panel shows histological sequence of epidermal edge advance (indicated by arrowsFig 3B

Fig. 3b. A) Control and db/db mice after wounding. B) Healing accelerated by MSC application in both control and db/db mice

In the experiments shown in Figures 3a and 3b, Dr. Falanga’s laboratory has found that MSCs were able to accelerate healing in the db/db mice. Also, the validity of the model is shown by the findings that db/db mice healed more slowly (p<0.5).

The mouse full-thickness tail wound model we have shown indicates the basic aspects of this model. We have successfully tested other mouse strains (C57BL/6, beige SCID, etc.). We have found that the baseline control mouse wounds require up to 20-21 days for healing.