| Project |
Paper |
| FTMS lab (O’Connor) |
 Quantitating the relative abundance of isoaspartyl residues in deamidated proteins by electron capture dissociation. Cournoyer JJ, Lin C, Bowman MJ, and O’Connor PB. J Am Soc Mass Spectrom 2007; 18(1): 48-56. |
| Project 10 (Steinberg/Farber) |
Identification of oxidative post-translational modification of serum albumin in patients with idiopathic pulmonary arterial hypertension and pulmonary hypertension of sickle cell anemia. Odhiambo A, Perlman DH, Huang H, Costello CE, Farber HW, Steinberg MH, McComb ME, Klings ES. Rapid Commun Mass Spectrom 2007; 21: 2195–2203. |
| Project 11 (Loscalzo/Leopold) |
Aldosterone impairs vascular reactivity by decreasing glucose-6-phosphate dehydrogenase activity. Leopold JA, Dam A, Maron BA, Scribner AW, Liao R, Handy DE, Stanton RC, Pitt B, Loscalzo J. Nature Med 2007; 13(2):189-197 doi:10.1038/nm1545. |
| Core lab (McComb) |
Proteomic mapping of stimulus-specific signaling pathways involved in THP-1 cells exposed to porphyromonas gingivalis or its purified components. Saba JA, McComb ME, Potts DL, Costello CE, Amar S. J Proteome Res 2007: 6 (6): 2211-2221. |
|
| FTMS (O’Connor) |
Removal of polyethylene glycols from protein samples using titanium dioxide. Zhao C and O’Connor PB. Anal Biochem 2007: doi:10.1016/j.ab.2007.03.024. |
| FTMS (O’Connor) |
Quantitative determination of isotope ratios from experimental isotopic distributions. Kaur P, O’Connor PB. Anal Chem 2007; 79: 1198-1204. |
| Projects 1, 2 (Cohen) |
Cysteine-674 of the sarco/endoplasmic reticulum calcium ATPase is required for the inhibition of cell migration by nitric oxide. Ying J, Tong XY, Pimentel DR, Weisbrod RM, Trucillo MP, Adachi T, Cohen RA. Arterioscler Thromb Vasc Biol 2007; 27: 783-790. [Epub ahead of print] PMID: 17234728. 2007 Jan 18. |
| Project 6 (Collins) |
A network biology approach to prostate cancer. Ergun A, Lawrence CA, Kohanski MA, Brennan TA, and Collins JJ. Mol Syst Biol 2007; 3:82. Open access via Molecular Systems Biology Article number 82; doi:10.1038/msb4100125. |
| Project 2 (Cohen) |
Quantification of oxidative post-translational modifications of cysteine thiols of p21ras associated with redox modulation of activity using isotope-coded affinity tags (ICAT) and mass spectrometry. Sethuraman M, Clavreul N, Huang H, McComb ME, Costello CE, Cohen RA. Free Rad Biol Med 2007: 42(6): 823-829. |
| Project 6 (Collins) |
Large-scale mapping and validation of Escherichia coli transcriptional regulation from a compendium of expression profiles. Faith JJ, Hayete B, Thaden JT, Mogno I, Wierzbowski J, et al. PLoS Biol 2007; 5(1): e8 doi:10.1371/journal.pbio.0050008. |
| Project 8 (Walsh) |
Adiponectin modulates inflammatory reactions via calreticulin receptor–dependent clearance of early apoptotic bodies. Takemura Y, Ouchi N, Shibata R, et al. J Clin Invest 2007; 117(2): 375-386. Article in BU Today Feb. 7, 2007. |
| Core lab (McComb) |
Coupling of protein HPLC to MALDI-TOF MS using an on-target device for fraction collection, concentration, digestion, desalting, and matrix/analyte cocrystallization. Perlman DH, Huang H, Dauly C, Costello CE, and McComb ME. Anal Chem 2007; Jan 26 Epub ahead of print. |
| Project 9 (Vasan) |
Gamma glutamyl transferase and metabolic syndrome, cardiovascular disease and mortality risk: The Framingham Heart Study. Arterioscler Thromb Vasc Biol 2007; 27:127-133, epublished Nov. 9, 2006. Lay summary |
| Core lab (McComb) |
Evaluation of an on-target sample preparation system for MALDI-TOF mass spectrometry in conjunction with normal-flow peptide HPLC for peptide mass fingerprint analyses. Rapid Commun Mass Spectrom 2007; 21:44-58. |
| FTMS (O’Connor) |
Use of a double resonance electron capture dissociation experiment to probe fragment intermediate lifetimes. J Amer Soc Mass Spectrom 2006; 17(11): 1605-1615. |
| Project 11 (Loscalzo, Leopold) |
Vascular calcification: Pathobiological mechanisms and clinical implications. Circ Res 2006; 99: 1044-1059.
Oxidative enzymopathies and vascular disease. Arterioscler Thromb Vasc Biol 2005; 25: 1332-1340. |
| Project 3 (Keaney) |
Contribution of clinical correlates and 13 C-reactive protein gene polymorphisms to interindividual variability in serum C-reactive protein level. Circulation 2006; 113: 1415-1423.
Circulating biomarkers in acute coronary syndromes: Something different or more of the same? Circulation 2005; 112: 778-780.
Hydrogen peroxide restrains endothelium-derived nitric oxide bioactivity—Role for iron-dependent oxidative stress. Free Radic Biol Med 2006; 41: 681–688. |
| Project 8 (Walsh) |
Impaired angiogenesis in glutathione peroxidase-1–deficient mice is associated with endothelial progenitor cell dysfunction. Circ Res 2006; 98: 254-261.
Microarray analysis of Akt1 activation in transgenic mouse hearts reveals transcript expression profiles associated with compensatory hypertrophy and failure. Physiol Genomics 2006: 27: 156-170. |
| Project 9 (Vasan) |
Biomarkers of cardiovascular disease: Molecular basis and practical considerations. Circulation 2006; 113 (19): 2335-2362.
Genome scan of systemic biomarkers of vascular inflammation in the Framingham Heart Study: Evidence for susceptibility loci on 1q. Atherosclerosis 2005; 182: 307–314.
Relations of inflammation and novel risk factors to valvular calcification. Am J Cardiol 2006; 97(10):1502-5. |
| Project 2 (Cohen) |
S-Glutathiolation of p21ras by peroxynitrite mediates endothelial insulin resistance caused by oxidized low-density lipoprotein Arterioscler Thromb Vasc Biol 2006 [published online Aug. 24, 2006 DOI: 0.1161/01.ATV.0000242791.28953.4c] |
| FTMS project (O’Connor) |
The effect of radical trap moieties on electron capture dissociation spectra of substance p. J Am Soc Mass Spectrom 2006; 17: 1428-1436.
Comparison of charge state determination methods for high resolution mass spectra. IEEE International Conference on Granular Computing 2006; 10-12 May 2006, pp 550 – 553. |
| Core Lab (McComb) |
Identification of transcription complexes that contain the double bromodomain protein Brd2 and chromatin remodeling machines. J Prot Res 2006; 5: 502-511. |
| Project 1,2 and Core Lab |
Preconditioning enters the era of “Physiological Proteomics”. Circ Res 2006; 99 (7): 663-665. |
| Project 12 (Colucci) |
Neuregulin activates erbB2-dependent src/FAK signaling and cytoskeletal remodeling in isolated adult rat cardiac myocytes. J Mol Cell Cardiol 2006: 41(2):228-35.
Role of reversible, thioredoxin-sensitive oxidative protein modifications in cardiac myocytes. Antioxid Redox Signal 2006; 8 (11-12): 2153-9.
Strain-stimulated hypertrophy in cardiac myocytes is mediated by reactive oxygen species-dependent Ras S-glutathiolation. J Mol Cell Cardiol 2006; 41: 613-622. |
| Project 4 (Loscalzo, Handy) |
Nitric oxide and post-translational modification of the vascular proteome. S-Nitrosation of reactive thiols. Arterioscler Thromb Vasc Biol 2006: 26(6):1207-14.
Aminoglycosides decrease glutathione peroxidase-1 activity by interfering with selenocysteine incorporation. J Biol Chem 2006; 281 (6): 3382-3388. |
| Project 9 (Vasan) |
Cross-sectional correlates of serum heat shock protein 70 in the community. Am J Hypertens 2006: 19(2): 227-231. |
| Core Laboratory (McComb) |
Protein separation and characterization by np-RP-HPLC followed by intact MALDI-TOF mass spectrometry and peptide mass mapping analyses. J Proteome Res 2006: 5(7): 1688-1700. |
| FTMS project (O’Connor) |
Detailed map of oxidative post-translational modifications of human P21Ras using Fourier Transform Mass Spectrometry. Anal Chem 2006; Web Release Date: 14-Jun-2006; (Article) DOI: 10.1021/ac060525v. |
| Project 1,2 (Cohen) |
Detection of sequence-specific tyrosine nitration of manganese SOD and SERCA in cardiovascular disease and aging. Am J Physiol Heart Circ Physiol 2006; 290: H2220-H2227. |
| Project 1,2 (Cohen) |
Glucose-6-phosphate dehydrogenase deficiency decreases vascular superoxide and atherosclerotic lesions in apolipoprotein E(-/-) mice. Arterioscler Thromb Vasc Biol 2006 Apr; 26(4): 910-6. Epub 2006 Jan 26. PMID: 16439706. |
| FTMS project (O’Connor) |
Algorithms for automatic interpretation of high resolution mass spectra. J Am Soc Mass Spectrom 2006; 17: 459-468. |
| FTMS project (O’Connor) |
Detecting deamidation products in proteins by electron capture dissociation. Anal Chem 2006; 78: 1264-1271. |
| Project 1,2 (Cohen) |
Nitric-oxide-induced vasodilatation: Regulation by physiologic S-glutathiolation and pathologic oxidation of the sarcoplasmic endoplasmic reticulum calcium ATPase. Trends Cardiovasc Med 2006: 16: 109-114. |
| FTMS project (O’Connor) |
Use of the filter diagonalization method in the study of space charge related frequency modulation in Fourier Transform Mass Spectrometry. J Am Soc Mass Spectrom 2006: 17 (4): 836-843. |
| FTMS project (O’Connor) |
Long-lived electron capture dissociation product ions experience radical migration via hydrogen abstraction. J Am Soc Mass Spectrom 2006: 17 (4): 576-585. |
| Project 1,2 (Cohen) |
The thromboxane receptor antagonist s18886 attenuates renal oxidant stress and proteinuria in diabetic apolipoprotein E-deficient mice. Diabetes 2006; 55 (1): 110-9. |
| FTMS project (O’Connor) |
Differentiation of aspartic and isoaspartic acids using electron transfer dissociation. J Am Soc Mass Spectrom 2006; 17: 15-19. |
| FTMS project (O’Connor) |
A new hybrid electrospray Fourier transform mass spectrometer: Design and performance characteristics. Rapid Commun Mass Spectrom 2006; 20: 259-266. Contact Peter O’Connor for reprints. |
| Project 2 (Cohen) |
S-glutathiolation by peroxynitrite of p21ras at cysteine-118 mediates its direct activation and downstream signaling in endothelial cells. FASEB J (express) published Jan. 13, 2006. |
| FTMS project (O’Connor) |
Characterization of a new qQq-FTICR mass spectrometer for post-translational modification analysis and top-down tandem mass spectrometry of whole proteins. J Am Soc Mass Spectrom 2005: 16: 1985-1999. |
| Project 1,2 (Cohen) |
Thromboxane A2 receptor antagonist, S18886, prevents enhanced atherogenesis caused by diabetes mellitus. Circulation 2005; 112: 3001-3008. Press release |
| Project 9 (Vasan) |
Homocysteine and heart failure: a review of investigations from the Framingham Heart Study. Clin Chem Lab Med 2005; 43 (10): 987–992. |
| Project 8 (Walsh) |
Impaired revascularization in a mouse model of type 2 diabetes is associated with the dysregulation of a complex angiogenic-regulatory network. Arterioscler Thromb Vasc Biol 2005; 25:1603-1609. |
| Project 3 (Keaney) |
p38 Mitogen-activated protein kinase activates eNOS in endothelial cells by an estrogen receptor alpha-dependent pathway in response to black tea polyphenols.Circ Res 2005; 96: 1072-1078. |
| Project 1,2 |
Glucose-6 phosphate dehydrogenase deficiency decreases the vascular response to angiotensin II. Circulation 2005; 112: 257-263. |
| FTMS project |
A minimum thickness gate valve with integrated ion optics for mass spectrometry. J Am Soc Mass Spectrom 2005; 16: 441-445. Contact Peter O’Connor for reprints. |
| Project 6 (Collins) |
Chemogenomic profiling on a genome-wide scale using reverse-engineered gene networks. Nature Biotechnology March 2005 Lay summary, abstract, press release University research spotlight |
| FTMS project |
Deamidation: Differentiation of aspartyl from isoaspartyl products in peptides by electron capture dissociation. Protein Sci Feb 2005 |
| Project 12 |
Alpha-adrenergic receptor-stimulated hypertrophy in adult rat ventricular myocytes is mediated via thioredoxin-1-sensitive oxidative modification of thiols on Ras. Circulation 2005; 111:1192-1198. Lay summary, abstract, press release University research spotlight |
| Core Laboratory collaboration |
S-nitrosoprotein formation and localization in endothelial cells. PNAS Jan 2005 Lay summary |
| Project 2 |
Isotope-coded affinity tag (ICAT) approach to redox proteomics: Identification and quantitation of oxidant-sensitive cysteine thiols in complex protein mixtures, J Proteome Research 2004; 3(6): 1228-1233. Lay summary |
| Core Laboratory collaboration |
A common open representation of mass spectrometry data and its application to proteomics research, Nature Biotechnology Nov 2004, a collaboration with the NHLBI-funded Proteomics Center at the Institute for Systems Biology, Ruedi Aebersold, P.I. |
| Project 2 |
S-Glutathiolation by peroxynitrite activates SERCA during arterial relaxation by nitric oxide Nature Med 2004; 10:1200-1207. Lay summary |
| FTMS project |
Coupling thin-layer chromatography with vibrational cooling MALDI Fourier Transform mass spectrometry for the analysis of ganglioside mixtures, Anal Chem Sept 2004 |
| Project 8 |
Augmentation of therapeutic angiogenesis using genetically-modified human endothelial progenitor cells with altered glycogen synthase kinase-3beta activity. J Biol Chem 2004; 279:49430-49438. |
| Project 3 |
Hypochlorous acid impairs endothelium-derived nitric oxide bioactivity through a superoxide-dependent mechanism Arterioscler Thromb Vasc Biol 2004: 24(11): 2028-2033. |
| FTMS project |
High pressure MALDI-FTMS: implications for proteomics, Int J Mass Spectrom 2004 |
| Project 9 |
Relations of plasma homocysteine to left ventricular structure and function: the Framingham Study, Eur Heart J 2004 |
| Projects 2, 12 |
S-Glutathiolation of Ras mediates redox-sensitive signaling by angiotensin II in vascular smooth muscle cells, J Biol Chem 2004 |
| FTMS |
Use of statistical methods for estimation of total number of charges in a mass spectrometry experiment, Analytical Chemistry 2004 |
| Project 9 |
Plasma homocysteine, hypertension incidence, and blood pressure tracking: The Framingham Heart Study, Hypertension 2003 |
| Project 2 |
Isotope-coded affinity tag approach to identify and quantify oxidant-sensitive protein thiols, Molecular and Cellular Proteomics 2004 |
| FTMS |
A high pressure MALDI FTMS ion source designed to accommodate large targets with diverse surfaces, JASMS 2004 (must be member of ASMS) |
| FTMS |
Attomole peptide analysis by high-pressure MALDI FTMS, Analytical Chemistry 2003 |
| Project 8 |
Akt signaling regulates side population cell phenotype via Bcrp1 translocation, J Biological Chemistry 2003 |
| Project 1 |
Quantitative assessment of tyrosine nitration of manganese superoxide dismutase in angiotensin II-infused rat kidney, American Journal of Physiology (Heart and Circulatory Physiology) 2003 |
| Project 3 |
Obesity and systemic oxidative stress: clinical correlates of oxidative stress in the Framingham Study, Arteriosclerosis, Thrombosis, and Vascular Biology 2003 |
