cryoFTMS Laboratory
First signal! reported in cryo FTMS lab.
The cryoFTMS laboratory charged the new magnet to 14 Tesla. This achievement made it the second highest magnetic field for FTICR in the world–second only to the 14.5 Tesla magnet at the National High Magnetic Field Laboratory in Tallahassee, Florida. This magnet has the potential of being charged to ~15 Tesla, which would make it the first. Under construction for over a year, it underwent rigorous performance tests by its manufacturer, Cryomagnetics, in facilities in Oak Ridge, Tennessee and in the cryoFTMS lab here and meets all specifications for field, homogeneity, and heat transfer. The magnet will be assembled into the next-generation Fourier Transform Mass Spectrometer, and will start to be used with research data shortly, meeting a major milestone of the Proteomics Initiative.
Cheng Zhao
Using FTMS instruments available at the NCRR-funded Mass Spectrometry Resource, researchers mapped oxidative stress post-translational modifications on p21Ras. Findings have been published in Analytical Chemistry (2006; 78: 5134-5142) demonstrating that this novel technology is useful for determination of very detailed information on protein structures. Cheng Zhao, Mahadevan Sethuraman, Nicolas Clavreul, Parminder Kaur, Richard Cohen, and Peter O’Connor authored the publication, a group that by its diversity shows the increasing levels of laboratory collaborations within and among the CPC research projects. The paper is entitled, “Detailed Map of Oxidative Post-translational Modifications of Human p21ras using Fourier Transform Mass Spectrometry,” available at: list of publications. Dr. Zhao, first author, who spent two years in the cryoFTMS lab, recently returned to Abbott Laboratories where she interned in 2004.
Vera Ivleva
Vera Ivleva, a graduate student, defended her thesis, “Direct coupling of thin layer chromatography with vibrationally cooled MALDI-MS for the analysis of glycolipids from biological samples,” April 12, 2007 and will continue in the lab. Early results from her studies were published in Analytical Chemistry in 2004. More on the cryoFTMS laboratory, led by Peter B. O’Connor, Ph.D., Research Associate Professor of Biochemistry…
