Zeiss LSM 510 Axiovert 200M Laser Scanning Confocal Microscope
The Zeiss LSM 510 Axiovert 200M is equipped with four argon laser lines (458, 477, 488, and 514 nm), two HeNe lasers (543 and 633 nm), and a 405 nm diode laser. Available objectives include 10X dry, 20X dry, 40X dry, 40X water, and 63X oil. Features supported by the LSM 510 software include single image acquisition, z-stacks, tiling, time-lapse imaging, and fluorescence recovery after photobleaching (FRAP). Additional features include software-based autofocusing and automated time-lapse imaging of multiple locations. An environmental chamber is available for maintaining cells at 37 °C and 5% carbon dioxide.
This instrument is a workhorse routinely used for live cell calcium imaging using a flow-through system and micromanipulator. These accessories are available upon discussion with Dr. Trinkaus-Randall.
External Academic Users Rates
External Commercial Users Rates
Persons are trained and then evaluated before working independently. Training usually requires about four hours, divided between two sessions.In some cases both parties decide that it is better for the imaging to be performed in an assisted manner.
Vickery Trinkaus-Randall, PhD
Tel: (617) 358-1235
- BUSM Instructional Building/(L building) Room L904
- BUSM Housman Medical Research Center / (R Building) Room R918