Zeiss LSM 510 Axiovert 200M Laser Scanning Confocal Microscope

LSM 510 zeiss axiovert 200m

The Zeiss LSM 510 Axiovert 200M is equipped with four argon laser lines (458, 477, 488, and 514 nm), two HeNe lasers (543 and 633 nm), and a 405 nm diode laser. Available objectives include 10X dry, 20X dry, 40X dry, 40X water, and 63X oil. Features supported by the LSM 510 software include single image acquisition, z-stacks, tiling, time-lapse imaging, and fluorescence recovery after photobleaching (FRAP). Additional features include software-based autofocusing and automated time-lapse imaging of multiple locations. An environmental chamber is available for maintaining cells at 37 °C and 5% carbon dioxide.

This instrument is a workhorse routinely used for live cell calcium imaging using a flow-through system and micromanipulator. These accessories are available upon discussion with Dr. Trinkaus-Randall.

BUMC Rates
  • $22/hour unassisted
  • $80/hour training/assisted
External Academic Users Rates
  • $50/hour unassisted
  • $100/hour training/assisted
External Commercial Users Rates
  • $100/hour unassisted
  • $140/hour training/assisted

Usage

Persons are trained and then evaluated before working independently. Training usually requires about four hours, divided between two sessions.In some cases both parties decide that it is better for the imaging to be performed in an assisted manner.

Sign up may be done by physically coming to L904 or emailing either vickery@bu.edu or kkneer@bu.edu for time.

Contact

Vickery Trinkaus-Randall, PhD
Core Director
Tel: (617) 638-5099
Email: vickery@bu.edu

Krisandra Kneer
Tel: (617) 638-4536
Email: kkneer@bu.edu

Locations

  • BUSM Instructional Building/(L building) Room L904
  • BUSM Housman Medical Research Center / (R Building) Room R918

Confocal Microscope Facility