Olympus DSU Wide-Field Spinning Disk Confocal Microscope

This system is good for:

  • Time lapse of fast events such as protein translocation.
  • Fixed or living samples
  • Subcellular imaging
  • DAPI, FITC, Alexa 488, TEXAS red, Alexa 594, Alexa 647, Cy5 and similar indicators.
  • Calcium imaging using fura 2 (non-confocal).
  • Dual emission for FRET indicators (CFP-YFP).

This system is not good for:

  • Very high resolution (less than 0.5 micron resolution)

Function of Instrument

This system is a relatively high-speed, medium sensitivity confocal.

Overview

The excitation source is a Prior Lumen pro 220 mercury halide wideband light source. A physiological incubation chamber can be used for live-cell imaging. The detector is a cooled sCMOS camera. It is ideal for capturing high-speed events such as calcium transients, rapid translocation events, and transient membrane potential changes. It is also suitable for fixed samples.

Training, Usage and Maintenance

  • Training for the use of this system involves familiarizing the user with the proper turn on and turn off sequences so as not to damage the equipment.
  • Maintenance involves optical alignment, cleaning optics, and replacing the light source bulb.
  • New users should schedule a training session of 2 hours. Please book your training 2 weeks in advance. Bring your sample to training and we will acquire images with you.

Please use the Cores Equipment Scheduler (CES) for scheduling, pricing and appointment information. For new users click here.

Help us help you.

Grants: The Cellular Imaging Core operates at a loss and is subsidized by the Department of Medicine. What does not get included in the balance sheets are the indirect costs generated from grants obtained with the help of data from our core. You can help us continue to serve you by letting us know when you submit a proposal and/or are awarded a grant which contains data obtained from the use of our Core.

Acknowledgments: We would greatly appreciate it if authors would acknowledge the Cellular Imaging Core in their publications containing data obtained with the equipment and/or assistance of Core personnel.

Location: 670 Albany Street, EBRC Building – Basement B15.

Contact:

Michael T. Kirber, PhD
Core Director
Office: (617) 638-7153
Cellular Imaging Core