Leica SP5 Confocal Microscope

Leica SP5 Confocal Microscope

Function of the instrument

Laser point scan confocal with standard and high-speed resonance scanning. High resolution imaging at room temperature.

Overview

The Leica SP5 is a point-scan confocal microscope with laser lines at 405, 458, 476,
488, 496, 514, 543, 594, and 633nm. Emission collection is customizable to 4 channels.
Regular and high-speed resonance scanning modes are available.

  • Training involves proper turn on and turn off sequences and learning the operation of the instrument.
  • Training also involves the proper cleaning so as not to damage the optics or other parts of the instrument.
  • Generally, the instructor is operating the instrument for the first session and explaining as the experiment progresses and is present for following sessions as needed.
  • New users should schedule at least 2 training sessions of 1 hour. Please book your training 2 weeks in advance. Bring your sample to training and we will acquire images with you.

Please use the Cores Equipment Scheduler (CES) for scheduling, pricing and appointment information. For new users click here.

Help us help you.

Grants: The Cellular Imaging Core operates at a loss and is subsidized by the Department of Medicine. What does not get included in the balance sheets are the indirect costs generated from grants obtained with the help of data from our core. You can help us continue to serve you by letting us know when you submit a proposal and/or are awarded a grant which contains data obtained from the use of our Core.

Acknowledgments: We would greatly appreciate it if authors would acknowledge the Cellular Imaging Core in their publications containing data obtained with the equipment and/or assistance of Core personnel.

Location: 670 Albany Street, EBRC Building – Basement B15.

Contact:

Michael T. Kirber, PhD
Core Director
Office: (617) 638-7153
Cellular Imaging Core