Immunohistochemistry (IHC) Service Center

Welcome to the Immunohistochemistry (IHC) Service Center. Our staffs are here to help you with all your IHC and immunofluorescence (IF) needs including experimental design, troubleshooting, protocol database, etc. We provide automated IHC and IF staining service, as well as equipment use for the preparation of tissue and cell slides.

The IHC Service Center is currently equipped with an IntelliPATH Autostainer that can perform automated IHC or IF staining of up to 50 different slides simultaneously. The total flexibility of this stainer provides a fast tool to optimize new antibodies, new tissues in as short as one day. Multiple users can perform different staining protocols at the same time. We are also equipped with a microtome, an embedding machine, a decloaker, and a hypercenter.

Please contact via email Xuemei Zhong, PhD  to get started and schedule a consultation. To schedule equipment time or services use the Cores Equipment Scheduler (CES). For new users click here.

Services and Instrument Use



Antigen retrieval using Biocare decloaker Evans Biomedical Research Center (EBRC) – Building X – 431 $25 per run (Up to 40 slides). Free for users using our IHC and IF services.
Automated tissue processing and paraffin tissue embedding EBRC/Bldg. X – 431 $3 per cassette (Minimum 10 cassettes per run)
Cryosectioning EBRC/Bldg. X – 431 $ 10 per slide
Microtome Sectioning EBRC/Bldg. X – 431 $10 per hour (User cut the tissue section. Price includes the use of microtome, water bath, slides-warmer)
Microtome sectioning training or assistance EBRC/Bldg. X – 431 $30 per session
H&E staining EBRC/Bldg. X – 431 $15 per slide
Automated Immunohistochemistry (IHC) and Immunoflorescent (IF) staining EBRC/Bldg. X – 431 $30 per slide (Price includes deparaffinization, antigen retrieval, counter staining. All reagents are included except for primary antibody or user specific reagent.)

Experiment design and trouble shooting consultation

EBRC/Bldg. X – 431 $75 per session

IHC slide imaging

EBRC/Bldg. X – 431 $25 per hour


Xuemei Zhong, PhD
Director & Scientific Consultant
Office: (617) 638-7028

Natalie Bitar, MPH 
Manager & IHC Specialist
Office: (617) 638-7003


Evans Biomedical Research Center
650 Albany Street, 4th Floor Office
Building X – Room 431 & 437
Boston, MA 02118