Shelley J. Russek, Ph.D.

Shelley

Professor of Pharmacology

Director, BU Graduate Program for Neuroscience
Ph.D.: Boston University School of Medicine

Laboratory: Laboratory of Translational Epilepsy

Research Interests of the Russek Laboratory

Gene Regulation in the Nervous System

Our chief interests surround a desire to understand the dynamic regulation of gene expression in the nervous system and how it relates to the etiology of neurological and neuropsychiatric disease. The identification of gene families with multiple genes that code for related yet distinct receptor isoforms has added a remarkable increase to the level of specificity and complexity that governs the transcriptional regulation of neurotransmitter receptors in the CNS. For instance, the diverse set of nineteen genes coding for the major inhibitory receptor in the brain, the GABA-A receptor, constitutes a gene family that displays an unusual degree of differential developmental and cell-specific gene regulation. Individual control over specific subunit gene expression leads to functional and pharmacological diversity that enables individual neurons to respond dynamically to alterations in membrane excitability via the alteration of the number or kind of synaptic and/or extrasynaptic inhibitory receptors. We employ a variety of techniques, including chromatin immunoprecipitation (ChIP), primary neuronal cultures, living promoter/reporter assays as visualized by confocal microscopy, and the use of inducible viral vectors in animal models of disease, such as epilepsy, autism and depression to test hypotheses of disease etiology and potential strategies for novel molecular therapeutics that restore the balance of inhibition and excitation in the brain.

We are currently expanding our methodologies to include the use of behavioral assays that monitor the effects of viral transgene or shRNA rescue on neuropathology that is relevant to the behaving and diseased organism. Finally, we are using novel computational strategies in collaboration with leaders in the field of bioinformatics and systems biology to identify gene regulatory pathways and unknown DNA regulatory elements that may be operative in the brain either for its normal function or important as contributors to the disease phenotype and we are developing novel viral vector technology for use by the neuroscience research community to give greater pharmacological control over the inducibility and temporal delivery of relevant RNAs in vivo.

Selected Publications

Lund IV, Hu Y, Yogendra SHR, Benham RS, Faris R, Russek SJ , Brooks-Kayal AR. BDNF Selectively Regulates GABAAA Receptor Transcription by Activation of the JAK/STAT Pathway. Science Signaling. 2008 October; 1(41): 1-14.

Kim J, Farb DH and Russek SJ (In Press) Promoter Structure. Encyclopedia of Neuroscience, Springer Publishing.

Hu Y, Russek SJ. BDNF and the diseased nervous system: a delicate balance between adaptive and pathological processes of gene regulation. J Neurochem. 2008 Apr; 105(1): 1-17. Epub 2008 Jan 18. Review.

Hu Y, Lund IV, Gravielle MC, Farb DH, Brooks-Kayal AR, Russek SJ. Surface expression of GABA (A) receptors is transcriptionally controlled by the interplay of CREB and its binding partner ICER. J Biol Chem. 2008 Apr 4; 283(14):9328-40. Epub 2008 Jan 7.

Mark KA, Quinton MS, Russek SJ, Yamamoto BK. Dynamic changes in vesicular glutamate transporter 1 function and expression related to methamphetamine-induced glutamate release. J Neurosci. 2007 Jun 20;27(25):6823-31.

Reddy TE, Shakhnovich BE, Roberts DS, Russek SJ, DeLisi C.. Positional clustering improves computational binding site detection and identifies novel cis-regulatory sites in mammalian GABAA receptor subunit genes. Nucleic Acids Res. 2007;35(3):e20. Epub 2007 Jan 3.

Raol YH, Lund IV, Bandyopadhyay S, Zhang G, Roberts DS, Wolfe JH, Russek SJ, Brooks-Kayal AR. Enhancing GABA(A) receptor alpha 1 subunit levels in hippocampal dentate gyrus inhibits epilepsy development in an animal model of temporal lobe epilepsy. J Neurosci. 2006 Nov 1;26(44):11342-6.

Gibbs TT, Russek SJ, Farb DH Sulfated steroids as endogenous neuromodulators. Pharmacol Biochem Behav. 2006 Aug;84(4):555-67. Epub 2006 Oct 4. Review.

Popik P, Kostakis E, Krawczyk M, Nowak G, Szewczyk B, Krieter P, Chen Z, Russek SJ, Gibbs TT, Farb DH, Skolnick P, Lippa AS, Basile AS. The anxioselective agent 7-(2-chloropyridin-4-yl)pyrazolo-[1,5-a]-pyrimidin-3-yl](pyridin-2-yl)methanone (DOV 51892) is more efficacious than diazepam at enhancing GABA-gated currents at alpha1 subunit-containing GABAA receptors. J Pharmacol Exp Ther. 2006 Dec;319(3):1244-52. Epub 2006 Sep 13.

Roberts DS, Hu Y, Lund IV, Brooks-Kayal AR, Russek SJ. Brain-derived neurotrophic factor (BDNF)-induced synthesis of early growth response factor 3 (Egr3) controls the levels of type A GABA receptor alpha 4 subunits in hippocampal neurons. J Biol Chem. 2006 Oct 6;281(40):29431-5. Epub 2006 Aug 10.

Roberts DS, Raol YH, Bandyopadhyay S, Lund IV, Budreck EC, Passini MJ, Wolfe JH, Brooks-Kayal AR, Russek SJ. (2005) Egr3 stimulation of GABRA4 promoter activity as a mechanism for seizure-induced up-regulation of GABAA receptor {alpha}4 subunit expression. Proc Natl Acad Sci U S A. 2005 Aug 16;102(33):11894-9. Epub 2005 Aug 9. [Abstract ]

Lippa A, Czobor P, Stark J, Beer B, Kostakis E, Gravielle M, Bandyopadhyay S, Russek SJ, Gibbs TT, Farb DH, Skolnick P. (2005) Selective anxiolysis produced by ocinaplon, a GABA(A) receptor modulator. Proc Natl Acad Sci U S A. 2005 May 17;102(20):7380-5. Epub 2005 May 3. [ Abstract ]

Gravielle MC, Faris R, Russek SJ, Farb DH. (2005) GABA induces activity dependent delayed-onset uncoupling of GABA/benzodiazepine site interactions in neocortical neurons. J Biol Chem. 2005 Jun 3;280(22):20954-60. Epub 2005 Apr 1. [ Abstract ]

Steiger JL, Bandyopadhyay S, Farb DH, Russek SJ. (2004) cAMP response element-binding protein, activating transcription factor-4, and upstream stimulatory factor differentially control hippocampal GABABR1a and GABABR1b subunit gene expression through alternative promoters. J Neurosci. 2004 Jul 7;24(27):6115-26. [ Abstract ]

Jang MK, Mierke DF, Russek SJ, Farb DH. (2004) A steroid modulatory domain on NR2B controls N-methyl-D-aspartate receptor proton sensitivity. Proc Natl Acad Sci U S A. 2004 May 25;101(21):8198-203. Epub 2004 May 18. [ Abstract ]

Martin SC, Steiger JL, Gravielle MC, Lyons HR, Russek SJ, Farb DH. (2004) Differential expression of gamma-aminobutyric acid type B receptor subunit mRNAs in the developing nervous system and receptor coupling to adenylyl cyclase in embryonic neurons. J Comp Neurol. 2004 May 17;473(1):16-29. [ Abstract ]

Steiger JL, Russek SJ. (2004) GABAA receptors: building the bridge between subunit mRNAs, their promoters, and cognate transcription factors. Pharmacol Ther. 2004 Mar;101(3):259-81. Review. Erratum in: Pharmacol Ther. 2004 Sep;103(3):261. [ Abstract ]

Lau GC, Saha S, Faris R, Russek SJ. (2004) Up-regulation of NMDAR1 subunit gene expression in cortical neurons via a PKA-dependent pathway. J Neurochem. 2004 Feb;88(3):564-75. [ Abstract ]

Steiger JL, Alexander MJ, Galler JR, Farb DH, Russek SJ. (2003) Effects of prenatal malnutrition on GABAA receptor alpha1, alpha3 and beta2 mRNA levels. Neuroreport. 2003 Sep 15;14(13):1731-5. [ Abstract ]

Steiger JL, Galler JR, Farb DH, Russek SJ. (2002) Prenatal protein malnutrition reduces beta(2), beta(3) and gamma(2L) GABA(A) receptor subunit mRNAs in the adult septum. Eur J Pharmacol. 2002 Jun 20;446(1-3):201-2. [ Abstract ]

Martin SC, Russek SJ, Farb DH. (2001) Human GABA(B)R genomic structure: evidence for splice variants in GABA(B)R1 but not GABA(B)R2. Gene. 2001 Oct 31;278(1-2):63-79. [ Abstract ]

Russek SJ, Bandyopadhyay S, Farb DH. (2000) An initiator element mediates autologous downregulation of the human type A gamma -aminobutyric acid receptor beta 1 subunit gene. Proc Natl Acad Sci U S A. 2000 Jul 18;97(15):8600-5. [ Abstract ]

McLean PJ, Shpektor D, Bandyopadhyay S, Russek SJ, Farb DH. (2000) A minimal promoter for the GABA(A) receptor alpha6-subunit gene controls tissue specificity. J Neurochem. 2000 May;74(5):1858-69. [ Abstract ]

Martin SC, Russek SJ, Farb DH. (1999) Molecular identification of the human GABABR2: cell surface expression and coupling to adenylyl cyclase in the absence of GABABR1. Mol Cell Neurosci. 1999 Mar;13(3):180-91. [ Abstract ]

Russek SJ. (1999) Evolution of GABA(A) receptor diversity in the human genome. Gene. 1999 Feb 18;227(2):213-22. [ Abstract ]

Yaghoubi N, Malayev A, Russek SJ, Gibbs TT, Farb DH. (1998) Neurosteroid modulation of recombinant ionotropic glutamate receptors. Brain Res. 1998 Aug 24;803(1-2):153-60. [ Abstract ]

Rabow LE, Russek SJ, Farb DH. (1995) From ion currents to genomic analysis: recent advances in GABAA receptor research. Synapse. 1995 Nov;21(3):189-274. Review. [ Abstract ]

McLean PJ, Farb DH, Russek SJ. (1995) Mapping of the alpha 4 subunit gene (GABRA4) to human chromosome 4 defines an alpha 2-alpha 4-beta 1-gamma 1 gene cluster: further evidence that modern GABAA receptor gene clusters are derived from an ancestral cluster. Genomics. 1995 Apr 10;26(3):580-6. [ Abstract ]

Russek SJ, Farb DH. (1995) Mapping of the beta 2 subunit gene of the GABAA receptor (GABRB2) to human chromosome 5q34 using fluorescence in situ hybridization. Cell Mol Biol Res. 1995;41(6):511-3. No abstract available. [ Abstract ]

Russek SJ, Farb DH. (1995) Mapping of the beta 2 subunit gene (GABRB2) to microdissected human chromosome 5q34-q35 defines a gene cluster for the most abundant GABAA receptor isoform. Genomics. 1994 Oct;23(3):528-33. [ Abstract ]

Farb, D. H., T.T. Gibbs, F.-S. Wu, M. Gyenes, L. Friedman and S.J. Russek (1992) Steroid Modulation of Amino Acid Receptors. In: Advances in Biochemical Psychopharmacology, V. 47: GABAergic Synaptic Transmission: Molecular, Pharmacological, and Clinical Aspects (G. Biggio, A. Concas, and E. Costa, eds.), pp. 119-131, Raven Press, New York.

Russek SJ, Quirk JC, Farb DH. (1993) Restriction selection cloning: a simple general method for the selection of recombinant DNA. Cell Mol Biol Res. 1993;39(2):177-82. [ Abstract ]

Farb DH, Gibbs TT, Wu FS, Gyenes M, Friedman L, Russek SJ. (1992) Steroid modulation of amino acid neurotransmitter receptors. Adv Biochem Psychopharmacol. 1992;47:119-31. No abstract available. [ Abstract ]

Russek LG, King SH, Russek SJ, Russek HI. (1990) The Harvard Mastery of Stress Study 35-year follow-up: prognostic significance of patterns of psychophysiological arousal and adaptation. Psychosom Med. 1990 May-Jun;52(3):271-85. [ Abstract ]

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Contact

Office: L-611
Phone: 617-638-4319; Fax: 617-638-4329
Lab phone: 617-638-5667
Email: srussek@bu.edu