Steven A. Bogen, MD, PhD

Adjunct Professor

Research Interests:

Dr. Bogen’s research group focuses on applied (translational) projects in the area of cancer detection and diagnosis, with an emphasis on new technology development.  As a result of this translational focus, more than a dozen U.S. patents were received.  Most of the patents relate to products that are now commercialized.  The first project was for the development of an automated instrument for performing special stains and immunohistochemistry testing.  The instrument staining system was completed in the late 1990’s and is called the “Artisan”, now sold by Dako Corporation.

More recently, the team developed a new technology for the more accurate molecular classification of solid tumors.  This is a laboratory testing technology for nationally standardized measurement of protein markers that are expressed by carcinomas.  Examples of tests used in the clinical management of breast carcinomas include Her-2, ER, and PR.  That technology, a system of controls and calibrators for quantitative immunohistochemistry, was funded by the National Cancer Institute.  The results of national clinical trials were published in 2008.  With the project complete, the technology was transferred to a commercial partner for large scale production, marketing, and distribution.

The group is currently working with a new platform discovery technology called Epitope-Mediated Antigen Prediction (E-MAP).  The group developed a method to identify the antigen to which a patient’s serum antibody binds without any other clues as to the antigen’s identity.  The method uses a peptide combinatorial library to test billions of different peptide combinations, to find the best fit to the antibody’s binding site.  The sequence is then matched to peptide sequences in the protein database.  This method provides important clues to the etiology of diseases of unknown origin, without requiring any pre-existing suspicion or clinical clues.  The method was published in Molecular and Cellular Proteomics, February 2008.  The group first applied this technology to the analysis of paraproteins from patients with multiple myeloma.  Those findings were published in Blood, January 2008.  E-MAP is expected to create new discovery insights and diagnostic opportunities in B-lymphoproliferative disorders, autoimmunity, certain granulomatous diseases of unknown origin, and infectious diseases.

Education:

  • M.D. University of Chicago Pritzker School of Medicine
  • Ph.D. Weizmann Institute of Science

Recent Representative Publications:

SA Bogen, K Vani, & SR Sompuram. 2008. Molecular Mechanisms of Antigen Retrieval: Antigen Retrieval Reverses Steric Interference Caused By Formalin-Induced Crosslinks.  Biotechnic & HistochemIn press.

G Bastas, SR Sompuram, B Pierce, K Vani, & SA Bogen.  2008.  Bioinformatic Requirements for Protein Database Searching Using Predicted Epitopes From Disease-Associated Antibodies.  Mol. Cell Proteomics. 7:247-256.

SR Sompuram, G Bastas, K Vani, SA Bogen. 2008.  Accurate Identification Of Paraprotein Antigen Targets By Epitope Reconstruction.  Blood. 111:302-308.

K Vani, SR Sompuram, & SA Bogen.  2008.  National HER-2 Proficiency Test Findings Using Standardized Quantitative Controls: Characterization of Laboratory Failures.  Arch. Pathol. Lab. Med. 132:211-216.

K Vani, SA Bogen, & SR Sompuram.  2006.  A High Throughput Combinatorial Library Technique for Identifying Formalin-Sensitive Epitopes.  J. Immunol. Methods 317(1-2):80-89.

Rosencranz, R & SA Bogen. 2006. Clinical laboratory measurement of serum, plasma, and blood viscosity. Pathology Patterns Reviews 125(Suppl 1): S78-S86.

Sompuram, SR, K Vani , LJ Hafer, & SA Bogen. 2006.  Antibodies Immunoreactive With Formalin-Fixed Tissue Antigens Recognize Linear Protein Epitopes. Am. J. Clin. Pathol. 125(1):82-90.

Sompuram, SR, K Vani , & SA Bogen. 2006.  A Molecular Model of Antigen Retrieval Using a Peptide Array.  Am. J. Clin. Pathol. 125(1):91-98.

Bogen, SA & SR Sompuram.  2004.  Recent Trends and Advances in Immunodiagnostics of Solid Tumors.  BioDrugs 18(6): 387-398.

Sompuram SR, Vani K., Wei L., Ramanathan H., Olken S., & Bogen SA. 2004. A water-stable protected isocyanate glass array substrate. Anal. Biochem. 326:55-68.

Sompuram SR. Vani K. Messana E. Bogen SA. 2004 A molecular mechanism of formalin fixation and antigen retrieval. American Journal of Clinical Pathology. 121(2):190-9.

Sompuram SR., McMahon D., Vani K., Ramanathan H., & Bogen SA. 2003. A novel microscope slide adhesive for poorly adherent tissue sections. J Histotechnol. 26(2):117-123.

Sompuram SR. Kodela V. Zhang K. Ramanathan H. Radcliffe G. Falb P. Bogen SA. A novel quality control slide for quantitative immunohistochemistry testing. Journal of Histochemistry & Cytochemistry. 50(11):1425-34, 2002 Nov.

Sompuram SR. Kodela V. Ramanathan H. Wescott C. Radcliffe G. Bogen SA. 2002. Synthetic peptides identified from phage-displayed combinatorial libraries as immunodiagnostic assay surrogate quality-control targets. Clinical Chemistry. 48(3):410-20.

Also, Dr. Bogen is listed as inventor or co-inventor on the following U.S. Patents:

7,217,392; 7,011,940; 6,783,733; 6,673,620; 6,541,261; 6,281,004; 6,183,693; 6,180,061; 6,096,271; 5,947,167; 5,645,114; 5,316,452; 5,073,504; 4,847,208

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