Deptartment of Biochemistry, Boston University, 670 Albany Street, Room 509, Boston, MA 02118, USA.

The biological significance of protein and lipid glycosylation is well established. For example, cells respond to environmental stimuli by altering glycan structures on their surfaces, and cancer cells evade normal growth regulation in part by remodeling their surface glycans. In general, glycan chemical properties differ significantly from those of proteins, lipids, nucleic acids, and small molecule metabolites. Thus, advances in glycomics, a comprehensive study to identify all glycans in an organism, rely on the development of specialized analytical methods. Mass spectrometry (MS) is emerging as an enabling technology in the field of glycomics. This review summarizes recent developments in mass spectrometric analysis methods for protein-based glycomics and glycoproteomics workflows.

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The award recognizes Prof. Costello’s contributions to proteomics. Here are links to some of Prof. Costello’s proteomics publications:

• Zhao, C.; Xie, B.; Chan, S. Y.; Costello, C. E.; O’Connor, P. B. Collisionally activated dissociation and electron capture dissociation provide complementary structural information for branched permethylated oligosaccharides. J Am Soc Mass Spectrom 2008, 19, 138-50. Link


• Lavatelli, F.; Perlman, D. H.; Spencer, B.; Prokaeva, T.; McComb, M. E.; Theberge, R.; Connors, L. H.; Bellotti, V.; Seldin, D. C.; Merlini, G.; Skinner, M.; Costello, C. E. Amyloidogenic and associated proteins in systemic amyloidosis proteome of adipose tissue. Mol Cell Proteomics 2008, 7, 1570-83. Link


• Sethuraman, M.; Clavreul, N.; Huang, H.; McComb, M. E.; Costello, C. E.; Cohen, R. A. Quantification of oxidative posttranslational modifications of cysteine thiols of p21ras associated with redox modulation of activity using isotope-coded affinity tags and mass spectrometry. Free radical biology & medicine 2007, 42, 823-9. Link


• Saba, J. A.; McComb, M. E.; Potts, D. L.; Costello, C. E.; Amar, S. Proteomic Mapping of Stimulus-Specific Signaling Pathways Involved in THP-1 Cells Exposed to …. J. Proteome Res 2007, 6, 2195-2204. Link


• Perlman, D. H.; Huang, H.; Dauly, C.; Costello, C. E.; McComb, M. E. Coupling of protein HPLC to MALDI-TOF MS using an on-target device for fraction collection, concentration, digestion, desalting, and matrix/analyte cocrystallization. Anal. Chem. 2007, 79, 2058-66. Link