Cheng Lin

Center for Biomedical Mass Spectrometry
Department of Biochemistry
Boston University School of Medicine
670 Albany Street, 5th floor
Boston, MA 02118-2526 USA

(617)-638-6705
(617)-638-6761 (FAX)
Email: Cheng Lin

Research Interests:

Dr. Lin’s group focuses on the development of mass spectrometry (MS) methods for the structural characterization of biomolecules. Specifically, they are interested in developing radical driven fragmentation methods that are amenable to the Fourier-transform ion cyclotron resonance mass spectrometry. One such method is the electron capture dissociation (ECD), which has several advantages over conventional slow heating methods. For instance, ECD retains labile side-chain modifications in proteins making it particularly suited for post-translational modification (PTM) analysis. ECD also produces extensive cross-ring cleavages in carbohydrates, which provides crucial information for studying complex glycan structure. Another tandem MS method that we are currently exploring is the vacuum ultraviolet photodissociation (VUV-PD). Unlike ECD, VUVPD is applicable to singly charged precursor ions that are commonly produced by the matrix-assisted laser desorption/ionization (MALDI). Current research efforts also include the application of these methods to solve important biological problems, such as protein deamidation, which plays an significant role in protein misfolding diseases and aging.

Publications:

  • Li, X.; Lin, C; O’Connor, P. B., Glutamine Deamidation: Differentiation of Glutamic Acid and gamma-Glutamic Acid in Peptides by Electron Capture Dissociation, Anal. Chem. 2010, 82, 3606.
  • Li, X.; Lin, C.; Han, L.; Costello, C. E.; O’Connor, P. B., Charge Remote Fragmentation in Electron Capture and Electron Transfer Dissociation, J. Am. Soc. Mass Spectrom. 2010, 21, 646.
  • Sargaeva, N. P.; Lin, C.; O’Connor, P. B., Identification of Aspartic and Isoaspartic Acid Residues in Amyloid β Peptides, Including Aβ1−42, Using Electron−Ion Reactions, Anal. Chem. 2009, 81, 9778.
  • Kong, X.; Lin, C.; Infusini, G.; Oh, H.-B.; Jiang, H.; Breuker, K.; Wu, C.-C.; Charkin, O. P.; Chang, H.-C.; McLafferty, F. W. ,Numerous Isomers of Serine Octamer Ions Characterized by Infrared Photodissociation Spectroscopy, ChemPhysChem 2009, 10, 2603.
  • Li, X.; Cournoyer, J. J.; Lin, C.; O’Connor, P. B., The Effect of Fixed Charge Modifications on Electron Capture Dissociation, J. Am. Soc. Mass Spectrom. 2008, 19, 1514.
  • Li, X.; Cournoyer, J. J.; Lin, C.; O’Connor, P. B., Use of 18O Labels to Monitor Deamidation during Protein and Peptide Sample Processing,  J. Am. Soc. Mass Spectrom. 2008, 19, 855.
  • Lin, C.; Cournoyer, J. J.; O’Connor, P. B.,  Probing the Gas Phase Folding Kinetics of Peptide Ions by IR Activated DR-ECD, J. Am. Soc. Mass Spectrom. 2008, 19, 780.
  • Lin, C.; Mathur, R.; Aizikov, K.; O’Connor, P. B., First Signal on the Cryogenic Fourier-Transform Ion Cyclotron Resonance Mass Spectrometer,  J. Am. Soc. Mass Spectrom. 2007, 18, 2090.
  • Cournoyer, J. J.; Lin, C.; Bowman, M. J.; O’Connor, P. B., Quantitating the Relative Abundance of Isoaspartyl Residues in Deamidated Proteins by Electron Capture Dissociation,  J. Am. Soc. Mass Spectrom. 2007, 18, 48.
  • Lin, C.; Cournoyer, J. J.; O’Connor, P. B., Use of a Double Resonance Electron Capture Dissociation Experiment to Probe Fragment Intermediate Lifetimes,  J. Am. Soc. Mass Spectrom. 2006, 17, 1605.
  • Belyayev, M. A.; Cournoyer, J. J.; Lin, C.; O’Connor, P. B., The Effect of Radical Trap Moieties on Electron Capture Dissociation Spectra of Substance P.  J. Am. Soc. Mass Spectrom. 2006, 17, 1428.
  • O’Connor, P. B.; Lin, C.; Cournoyer, J. J., Long-lived electron capture dissociation product ions experience radical migration via hydrogen abstraction,  J. Am. Soc. Mass Spectrom. 2006, 17, 576.
  • Cournoyer, J. J.; Lin, C.; O’Connor, P. B., Detecting Deamidation Products in Proteins by Electron Capture Dissociation,  Anal. Chem. 2006, 78, 1264.
  • Zabrouskov, V.; Han, X.; Welker, E.; Zhai, H.; Lin, C.; van Wijk, K. J.; Scheraga, H. A.; McLafferty, F. W., Stepwise Deamidation of Ribonuclease A at Five Sites Determined by Top Down Mass Spectrometry,  Biochem. 2006, 45, 987.
  • O’Connor, P. B.; Pittman, J. L.; Thomson, B. A.; Budnik, B. A.; Cournoyer, J. J.; Jebanathirajah, J.; Lin, C.; Moyer, S.; Zhao, C., A New Hybrid Electrospray Fourier Transform Mass Spectrometer: Design and Performance Characteristics,   Rapid Commun. Mass. Spectrom. 2006, 20, 259.
  • Oh, H.-B.; Lin, C.; Hwang, H. Y.; Zhai, H.; Breuker, K.; Zabrouskov, V.; Carpenter, B. K.; McLafferty, F. W., Infrared Photodissociation Spectroscopy of Electrosprayed Ions in a Fourier-Transform Mass Spectrometer.  J. Am. Chem. Soc. 2005, 127, 4076.
  • Breuker, K.; Oh, H.-B.; Lin, C.; Carpenter, B. K.; McLafferty, F. W., Nonergodic and Conformational Control of the Electron Capture Dissociation of Protein Cations,  Proc. Nat. Acad. Sci. USA 2004, 101, 14011.
  • Lin, C.; Witinski, M. F.; Davis, H. F., Oxygen Atom Rydberg Time-of-Flight Spectroscopy,   J. Chem. Phys. 2003, 119, 251.
  • Strazisar, B. R.; Lin, C.; Davis, H. F.,  Vibrationally Inelastic Scattering of High-n Rydberg H Atoms from N2 and O2,  Phys. Rev. Lett. 2001, 86, 3997.
  • Strazisar, B. R.; Lin, C.; Davis, H. F., Mode Specific Energy Disposal in the 4-Atom Reaction OH + D2 -> HOD + D,  Science 2000, 290, 958.
Primary teaching affiliate
of BU School of Medicine