Director, Cell and Molecular Biology Graduate Program
Boston University School of Medicine
School of Medicine Instructional Building, L903
715 Albany Street
Boston, MA 02118
Lab Phone: 617-638-4536
Joint Appointment: Professor of Ophthalmology
AB, Kenyon College, Gambier, OH
PhD, University of Wisconsin, Madison, WI
Our laboratory is interested in the wound response and repair of epithelium, neurons and extracellular matrix. 1. In the healthy cornea there are no blood vessels and thus the tissue provides an excellent model to study the communication between nerves and epithelium following injury. Primary neurons are co-cultured with epithelial cells to ask how signaling pathways communicate. These include but are not limited to glutamatergic and purinergic receptor-mediated calcium transients. 2. In addition to these signaling events we have found that when epithelial cells are injured nucleotides are released immediately and cause a distinct phosphorylation of EGF receptor residues that ultimately affect the ability of a cell to migrate. 3. To study controlling factors on extracellular matrix or stromal formation we have developed scaffold-free long-term cultures and these have recently enabled us to study the role of hypoxia, which occurs in a diurnal pattern in this tissue as one sleeps. Our experiments are conducted using a number of organ culture and cell models and use biochemical, cell and molecular techniques. 4. Last but not least we are interested in the role of long sugar chains or glycosaminoglycans on the formation of oligomers and fibrils in light chain amyloid disease using atomic force microscopy, negative staining and biochemical technologies.
- New insights in wound response and repair of epithelium. Chi C, Trinkaus-Randall V. J Cell Physiol. 2013 May;228(5):925-9. doi: 10.1002/jcp.24268.
- Communication between corneal epithelial cells and trigeminal neurons is facilitated by purinergic (P2) and glutamatergic receptors. Oswald DJ, Lee A, Trinidad M, Chi C, Ren R, Rich CB, Trinkaus-Randall V. PLoS One. 2012;7(9):e44574. doi: 10.1371/journal.pone.0044574. Epub 2012 Sep 7.
- Disorganized collagen scaffold interferes with fibroblast mediated deposition of organized extracellular matrix in vitro. Saeidi N, Guo X, Hutcheon AE, Sander EA, Bale SS, Melotti SA, Zieske JD, Trinkaus-Randall V, Ruberti JW. Biotechnol Bioeng. 2012 Oct;109(10):2683-98. doi: 10.1002/bit.24533. Epub 2012 May 4.
- Mankus C, Chic C, Ren R, Rich CB and Trinkaus-Randall V. The P2X7 Receptor Regulates Proteoglycan Expression in the Corneal Stroma. Molecular Vision. 2012; 18:128-138.
- Mankus C, Rich CB, Minns M, Trinkaus-Randall V. Corneal Epithelium Expresses a Variant of P2X(7) Recetpor in Health and Disease. PLoS One. 2011; 6(12):e28541. Epub 2011, Dec. 6.
- Ward JE, Ren R, Toraldo G, Soohoo P, Guan J, O’Hara C, Jasuja R, Trinkaus-Randall V, Liao R, Connors LH, Seldin DC. Doxycycline reduces fibril formation in a transgenic mouse model of AL amyloidosis. Blood. 2011. Dec 15; 118(25):6610-7. Epub 2011, Oct. 12.
- Karamichos D, Rich C, Hutcheon A, Ren R, Saitta B, Trinkaus-Randall V, Zieske J. Self-Assembled Matrix by Umbilical Cord Stem Cells. J. Functional Biomaterials (Biomaterials in Wound Healing) 2011, 2(3), 213-229; doi:10.3390/jfb20302013.
- Boucher I, Kehasse A, Marcincin M, Rich C, Rahimi N, Trinkaus-Randall V. Distinct Activation of Edpidermal Growth Factor Receptor by UTP Contributes to Epithelial Cell Wound Repair. Am J. Pathol. 2011 Mar;178(3):1092-105. PMID: 21356361 [PubMed - in process].
- Karamichos D, Rich C, Hutcheon A, Ren R, Saitta B, Trinkaus-Randall V, Zieske J. Self-Assembled Matrix by Umbilical Cord Stem Cells. J. Functional Biomaterials. 2011. 2:213-229.
- Ren R, Hong Z, Gong H, Laporte K, Skinner M, Seldin DC, Costello CE, Connors LH, Trinkaus-Randall V. The role of glycosaminoglycan sulfation in the formation of immunoglobulin light chain amyloid oligomers and fibrils. J Biol Chem. 2010 [Epub ahead of print] PMID: 20870723 [PubMed - as supplied by publisher]Free Article
- Boucher I, Rich C, Lee A, Marcincin M, Trinkaus-Randall V. The P2Y2 receptor mediates the epithelial injury response and cell migration. Am J Physiol Cell Physiol. 2010 Aug;299(2):C411-21. Epub 2010 Apr 28.PMID: 20427708 [ PubMed - indexed for MEDLINE]
- Wang X, Belguise K, O’Neill CF, Sánchez-Morgan N, Romagnoli M, Eddy SF, Mineva ND, Yu Z, Min C, Trinkaus-Randall V, Chalbos D, Sonenshein GE. RelB NF-kappaB represses estrogen receptor alpha expression via induction of the zinc finger protein Blimp1. Mol Cell Biol. 2009 Jul;29(14):3832-44. Epub 2009 May 11. PMID: 19433448 [PubMed - indexed for MEDLINE]
- Mayo C, Ren R, Rich C, Stepp MA and Trinkaus-Randall V, Regulation by P2X7: Epithelial migration and stromal organization in the cornea. Inv Ophthal Vis Sci. 49: 4384-4391. 2008.
- Ren R, Hutcheon AEK, Guo XQ, Melotti S, Ruberti JW, Zieske JD and Trinkaus-Randall V. Human primary corneal fibroblasts synthesize and deposit proteoglycans in longterm 3_D cultures. Dev Dyn. 237L2705-2715. 2008.
- Guo X, Hutcheon A, Melotti S, Zieske JD, Trinkaus-Randall V, Ruberti JW. Morphological Characterization of Organized Extracellular Matrix Deposition by Ascorbic Acid-Stimulated Human Corneal Fibroblasts. IOVS. 48:4050-60.2007
- Boucher I, Yang LL, Mayo C, Klepeis V and Trinkaus-Randall V. Injury and nucleotides induce phosphorylation of Epidermal Growth Factor Receptor:MMP and HB-EGF Dependent Pathway. Experimental Eye Res. 85:130-41E pub ahead of print. 2007.
- Monis, G, Schultz C, Ren R, Eberhard J, Costello E.C, Connors L, Skinner,M and Trinkaus-Randall V. Role of endocytic inhibitory drugs on internalization of amyloidogenic light chains by cardiac fibroblasts. Amer J Pathol. 169 (6) 1939-52, 2006
Selected Invited Chapters
- Cornell-Bell A, Jung P, Trinkaus-Randall V. Decoding Calcium Wave Signaling. Vol 31. pp. 661-689. In Advances in Molecular and Cell Biology. Elsevier Science, 2003 Ruberti J., Zieske JD., and V. Trinkaus-Randall.: Corneal Tissue Replacement in: The Principles of Tissue Engineering. Ch. 68. ed. Lanza, langer and Vacanti. Elsevier. 2007.
- Jung, P., Cornell-Bell A.H., Dreher M., deGrauw A., Strawsburg R., Trinkaus-Randall V.: Statistical analysis and modeling in calcium waves in healthy and pathological astrocyte syncytia. In Stochastic Processes in Physics, Chemistry and Biology. Springer Verlag, 2001.
- Trinkaus-Randall,V.: Cornea: Biological Responses. Ch. 35 pp 471-491 In: Principles of Tissue Engineering (second edition). eds. R. Lanza and R. Langer and E. Chick . Academic Press, Calif. 2000.