Matthew D. Layne
Assistant Professor
Silvio Conte Building, Office K203, Lab K219
72 E. Concord Street
Boston, MA 02118
Phone: 617-638-4361
Lab Phone: 617-638-5926
Fax: 617-638-5339
Email: mlayne
Education
BA, Boston University, Boston, MA
PhD, Boston University, Boston, MA
Postdoctoral Training: Brigham and Women’s Hospital/Harvard Medical School
People
| Dahai Wang Postdoc |
Ankur Prasad Graduate Student |
Kathleen Tumelty Graduate Student |
| Mike Jager Graduate Student |
Former Members here
Research Interests
Dr. Layne established his laboratory in the Biochemistry Department at Boston Univeristy Medical School in the summer of 2007.
Our long-term goals are to understand the transcriptional control of genes, which are upregulated in the vascular smooth muscle cells and fibroblasts in cardiovascular and pulmonary disease. We have identified a secreted protein, aortic carboxypeptidase-like protein (ACLP), which contains a collagen-binding discoidin and a catalytically inactive metallocarboxypeptidase domain. ACLP is expressed in vascular smooth muscle cells and is induced in the diseased blood vessel in vivo. We are defining the mechanisms by which ACLP regulates VSMC proliferation and function using in vitro assays and vascular disease models with transgenic and knockout mice. The lab is also investigating the control of myofibroblast gene expression in pulmonary fibrosis. We are exploring the mechanisms of gene expression and differentiation mechanisms in myofibroblasts and have identified novel signaling pathways and regulatory pathways that may coordinate the expression and subsequent deposition of extracellular matrix proteins in wounded VSMC and fibroblasts.
Current Projects:
- Investigating the regulation of genes in vascular smooth muscle cells in response to injury
- Determining the role of aortic carboxypeptidase-like protein in vascular smooth muscle biology
- Defining mechanism of fibroblast/myofibroblast transitions in fibrotic disease
Opportunities:
We are currently seeking graduate students. Please contact Dr. Layne by email for more information about research rotatations and projects.
Research Themes
Representative Publications
- Wang D, Prakash J, Nguyen P, Davis-Dusenbery BN, Hill NS, Layne MD, Hata A, Lagna G. Bone Morphogenetic Protein signaling in vascular disease: anti-inflammatory action through Myocardin-related transcription factor A. J. Biol. Chem. published 20 June 2012, 10.1074/jbc.M112.379487
- Luchsinger LL, Patenaude CA, Smith BD, Layne MD. Myocardin Related Transcription Factor-A Complexes Activate Type I Collagen Expression in Lung Fibroblasts. J Biol Chem 2011. 286(51) 44116-44125.
- Zhou X, Baron RM, Hardin M, Cho MH, Zielinski J, Hawrylkiewicz I, Sliwinski P, Hersh CP, Mancini JD, Lu K, Thibault D, Donahue AL, Klanderman BJ, Rosner B, Raby BA, Lu Q, Geldart AM, Layne MD, Perrella MA, Weiss ST, Choi AM, Silverman EK. Identification of a chronic obstructive pulmonary disease genetic determinant that regulates HHIP. Hum Mol Genet. 2011 Dec 2. [Epub ahead of print]
- Davis-Dusenbery BN, Chan MC, Reno KE, Weisman AS, Layne MD, Lagna G, Hata A. Downregulation of KLF4 by MIR-143/145 is critical for modulation of vascular smooth muscle cell phenotype by TGF-{beta} and BMP. J Biol Chem. 2011 286(32) 28097-110.
- Baron RM, Lopez-Guzman S, Riascos DF, Macias AA, Layne MD, Cheng G, Harris C, Chung SW, Reeves R, von Andrian UH, Perrella MA, Distamycin A inhibits HMGA1-binding to the P-selectin promoter and attentuates lung and liver inflammation during murine endotoxemia. PLoS One 5(5):e10656.
- Danzer E, Layne MD, Auber F, Shegu S, Kreiger P, Radu A, Volpe M, Adzick NS, Flake AW. Gastroschisis in mice lacking aortic carboxypeptidase-like protein (ACLP) is associated with a defect in neuromuscular development of the eviscerated intestine. Pediatr Res. 2010 68(1):23-8.
- Chen C-H, Wu M-L, Lee Y-C, Layne MD, Yet S-F. An Intronic CArG Box Regulates Cysteine-rich Protein 2 Expression in the Adult but not Developing Vasculature. Arterioscler Thromb Vasc Biol. 2010 30(4)835-842.
- Schissel SL, Dunsmore SE, Liu X, Shine RW, Perrella MA, Layne MD. Aortic carboxypeptidase-like protein is expressed in fibrotic human lung and its absence protects against bleomycin-induced lung fibrosis. Am J Pathol 2009;174(3):818-828.
- Liu X, Ramjiganesh T, Chen Y-H, Chung SW, Hall SR, Schissel SL, Padera RF, Liao R, Ackerman KG, Kajstura J, Leri A, Anversa P, Yet S-F, Layne MD, Perrella MA. Disruption of striated preferentially expressed gene locus leads to dilated cardiomyopathy in mice. Circulation, 2009; 119(2)261-8.
- Grant MA, Baron RM, Macias AA, Layne MD, Perrella MA, Rigby AC. Netropsin improves survival from endotoxemia by disrupting HMGA1-binding to the NOS2 promoter. Biochem J. 2009; 418(1):103-12.
- Lin D-W, Chang I-C, Tseng A, Wu M-L, Chen C-H, Patenaude CA, Layne MD, Yet S-F. Transforming growth factor β up-regulates cysteine-rich protein 2 in vascular smooth muscle cells via activating transcription factor 2. J Biol Chem. 2008;283(22):15003-14.
- Wei J, Gorman TE, Liu X, Ith B, Chen Z, Simon DI, Layne MD, Yet S-F. Vascular injury increases neointima formation in cysteine-rich protein 2-deficient mice. Circ Res 2005; 97:1323-1331.
- Ith B, Wei J, Yet S-F, Perrella MA, Layne MD. Aortic carboxypeptidase-like protein is expressed in collagen-rich tissues during mouse embryonic development. Gene Expr Patterns 2005;5(4):533-537.
- Layne MD, Yet S-F, Maemura K, Hsieh C-M, Liu X, Ith B, Lee M-E, Perrella MA. Characterization of the mouse aortic carboxypeptidase-like protein promoter reveals activity in both differentiated and dedifferentiated vascular smooth muscle cells. Circ Res 2002;90:728-36.
- Layne MD, Yet S-F, Hsieh C-M, Maemura K, Bernfield M, Perrella MA, Lee M-E. Impaired abdominal wall development and deficient wound healing in mice lacking aortic carboxypeptidase-like protein. Mol Cell Biol 2001;21:5256-61.
- Layne MD, Endege WO, Jain MK, Yet S-F, Hsieh C-M, Chin MT, Perrella MA, Blanar MA, Haber E, Lee M-E. Aortic carboxypeptidase-like protein, a novel protein with discoidin and carboxypeptidase-like domains, is up-regulated during vascular smooth muscle cell differentiation. J Biol Chem 1998;273:15654-60.
