Matthew D. Layne

ML.mug.
Associate Professor

Silvio Conte Building, Office K203, Lab K219
72 E. Concord Street
Boston, MA 02118

Phone: 617-638-4361
Lab Phone: 617-638-5926
Fax: 617-638-5339
Email:
Matthew Layne

Education

BA, Boston University, Boston, MA
PhD, Boston University, Boston, MA
Postdoctoral Training: Brigham and Women’s Hospital/Harvard Medical School

BU Profiles

People

Dahai Wang
Postdoctoral Associate
Mike Jager
Graduate Student
Carl Ceraolo
Graduate Student
Anupama Tiwari
Postdoctoral Associate
Ana de la Cueva
Postdoctoral Associate

Former Members here

Teaching

Dr. Layne directs 2 graduate level courses:
GMS BI777 Techniques in Biochemistry, Cell, and Molecular Biology: More info here BI777
GMS FC762 Critical Thinking in Biomedical Research: More info here FC762

Research Interests

The primary goal of our laboratory is to identify novel pathways that control fibroproliferation with the goal of developing therapeutic inhibitors. Fibroproliferative responses are similar to wound healing processes involving accumulation of contractile myofibroblasts and extracellular matrix (ECM) secretion. Because organ fibrosis, cardiovascular, metabolic/obesity, and cancer pathologies are now recognized to be impacted by fibroblast-myofibroblast differentiation and ECM remodeling our research is examining novel pathways and control mechanisms in these diseases. Central to our studies is determining the function of Aortic carboxypeptidase-like Protein (ACLP), a secreted, collagen-binding protein that enhances fibrosis and myofibroblast differentiation through mechanisms that involve stimulating the transforming growth factor β (TGFβ) receptor signaling complex and controlling mechanical signaling and ECM remodeling.

ACTIVE RESEARCH PROJECTS:

1. Regulation of vascular smooth muscle cell and adventitial fibroblast differentiation and remodeling.

2. Control of lung and skin fibrosis.

3. Development of tumor stroma directed therapies.

4. Regulation of adipose stem cell differentiation and adipose tissue fibrosis.

Research Themes

Cell Biology
Inflammation & ECM
Metabolism

Representative Publications

PubMed Search

  • Shiwen X, Stratton R, Nikitorowicz-Buniak J, Ahmed-Abdi B, Ponticos M, Denton C, Abraham D, Takahashi A, Suki B, Layne MD, Lafyatis R, Smith BD. A Role of Myocardin Related Transcription Factor-A (MRTF-A) in Scleroderma Related Fibrosis. PLoS One. 2015; 10(5):e0126015.
  • McDonald ME, Li C, Bian H, Smith BD, Layne MD*, Farmer SR*. Myocardin-related transcription factor a regulates conversion of progenitors to beige adipocytes. Cell. 2015 Jan 15; 160(1-2):105-18. PubMed *communicating
  • Xu YX, Ashline D, Liu L, Tassa C, Shaw SY, Ravid K, Layne MD, Reinhold V, Robbins PW. The glycosylation-dependent interaction of perlecan core protein with LDL: implications for atherosclerosis. J Lipid Res. 2015 Feb; 56(2):266-76. PubMed
  • Chen CH, Ho HH, Wu ML, Layne MD, Yet SF. Modulation of cysteine-rich protein 2 expression in vascular injury and atherosclerosis. Mol Biol Rep. 2014 Nov; 41(11):7033-41. PubMed
  • Wu ML, Chen CH, Lin YT, Jheng YJ, Ho YC, Yang LT, Chen L, Layne MD, Yet SF. Divergent signaling pathways cooperatively regulate TGFß induction of cysteine-rich protein 2 in vascular smooth muscle cells. Cell Commun Signal. 2014; 12:22. PubMed
  • Aortic Carboxypeptidase-like Protein Enhances Lung Myofibroblast Differentiation Through Transforming Growth Factor β Receptor Dependent and Independent Pathways. Tumelty KE, Smith BD, Nugent MA, Layne MD. J Biol Chem. 2014 Jan 31;289(5):2526-36. PMID: 24344132
  • Cysteine-rich protein 2 alters p130Cas localization and inhibits vascular smooth muscle cell migration. Chen CH, Ho YC, Ho HH, Chang IC, Kirsch KH, Chuang YJ, Layne MD, Yet SF. Cardiovasc Res. 100(3):461-71.
  • Wang D, Prakash J, Nguyen P, Davis-Dusenbery BN, Hill NS, Layne MD, Hata A, Lagna G. Bone Morphogenetic Protein signaling in vascular disease: anti-inflammatory action through Myocardin-related transcription factor A. J. Biol. Chem. published 20 June 2012, 10.1074/jbc.M112.379487
  • Luchsinger LL, Patenaude CA, Smith BD, Layne MD. Myocardin Related Transcription Factor-A Complexes Activate Type I Collagen Expression in Lung Fibroblasts. J Biol Chem 2011. 286(51) 44116-44125.
  • Zhou X, Baron RM, Hardin M, Cho MH, Zielinski J, Hawrylkiewicz I, Sliwinski P, Hersh CP, Mancini JD, Lu K, Thibault D, Donahue AL, Klanderman BJ, Rosner B, Raby BA, Lu Q, Geldart AM, Layne MD, Perrella MA, Weiss ST, Choi AM, Silverman EK. Identification of a chronic obstructive pulmonary disease genetic determinant that regulates HHIP. Hum Mol Genet. 2011 Dec 2. [Epub ahead of print]
  • Davis-Dusenbery BN, Chan MC, Reno KE, Weisman AS, Layne MD, Lagna G, Hata A. Downregulation of KLF4 by MIR-143/145 is critical for modulation of vascular smooth muscle cell phenotype by TGF-{beta} and BMP. J Biol Chem. 2011 286(32) 28097-110.
  • Baron RM, Lopez-Guzman S, Riascos DF, Macias AA, Layne MD, Cheng G, Harris C, Chung SW, Reeves R, von Andrian UH, Perrella MA, Distamycin A inhibits HMGA1-binding to the P-selectin promoter and attentuates lung and liver inflammation during murine endotoxemia. PLoS One 5(5):e10656.
  • Danzer ELayne MDAuber FShegu SKreiger PRadu AVolpe MAdzick NSFlake AW. Gastroschisis in mice lacking aortic carboxypeptidase-like protein (ACLP) is associated with a defect in neuromuscular development of the eviscerated intestine. Pediatr Res. 2010 68(1):23-8.
  • Chen C-H, Wu M-L, Lee Y-C, Layne MD, Yet S-F. An Intronic CArG Box Regulates Cysteine-rich Protein 2 Expression in the Adult but not Developing Vasculature. Arterioscler Thromb Vasc Biol. 2010  30(4)835-842.
  • Schissel SL, Dunsmore SE, Liu X, Shine RW, Perrella MA, Layne MD. Aortic carboxypeptidase-like protein is expressed in fibrotic human lung and its absence protects against bleomycin-induced lung fibrosis. Am J Pathol 2009;174(3):818-828.
  • Liu X, Ramjiganesh T, Chen Y-H, Chung SW, Hall SR, Schissel SL, Padera RF, Liao R, Ackerman KG, Kajstura J, Leri A, Anversa P, Yet S-F, Layne MD, Perrella MA. Disruption of striated preferentially expressed gene locus leads to dilated cardiomyopathy in mice. Circulation, 2009; 119(2)261-8.
  • Grant MA, Baron RM, Macias AA, Layne MD, Perrella MA, Rigby AC. Netropsin improves survival from endotoxemia by disrupting HMGA1-binding to the NOS2 promoter. Biochem J. 2009; 418(1):103-12.
  • Lin D-W, Chang I-C, Tseng A, Wu M-L, Chen C-H, Patenaude CA, Layne MD, Yet S-F. Transforming growth factor β up-regulates cysteine-rich protein 2 in vascular smooth muscle cells via activating transcription factor 2. J Biol Chem. 2008;283(22):15003-14.
  • Wei J, Gorman TE, Liu X, Ith B, Chen Z, Simon DI, Layne MD, Yet S-F. Vascular injury increases neointima formation in cysteine-rich protein 2-deficient mice. Circ Res 2005; 97:1323-1331.
  • Ith B, Wei J, Yet S-F, Perrella MA, Layne MD. Aortic carboxypeptidase-like protein is expressed in collagen-rich tissues during mouse embryonic development. Gene Expr Patterns 2005;5(4):533-537.
  • Layne MD, Yet S-F, Maemura K, Hsieh C-M, Liu X, Ith B, Lee M-E, Perrella MA. Characterization of the mouse aortic carboxypeptidase-like protein promoter reveals activity in both differentiated and dedifferentiated vascular smooth muscle cells. Circ Res 2002;90:728-36.
  • Layne MD, Yet S-F, Hsieh C-M, Maemura K, Bernfield M, Perrella MA, Lee M-E. Impaired abdominal wall development and deficient wound healing in mice lacking aortic carboxypeptidase-like protein. Mol Cell Biol 2001;21:5256-61.
  • Layne MD, Endege WO, Jain MK, Yet S-F, Hsieh C-M, Chin MT, Perrella MA, Blanar MA, Haber E, Lee M-E. Aortic carboxypeptidase-like protein, a novel protein with discoidin and carboxypeptidase-like domains, is up-regulated during vascular smooth muscle cell differentiation. J Biol Chem 1998;273:15654-60.