Analytical Services

1) Lipid (Fatty Acids) Analysis with GC/MS1

The Analytical Instrumentation Core (AIC) is now offering Fatty Acid analysis where we can detect and quantify both free and total fatty acids through the use of our HP 6890/5973 Gas Chromatograph and Mass Spectrometer (GC/MS).  Our GC/MS is designed for analyzing small molecules (up to 1,000 Da) such as fatty acids, lipids, monosaccharides, amino acids and neurotransmitters.

Currently, we are able to analyze the following sample types:*

    1. Plasma
    2. Blood
    3. Cell lines
    4. Tissues
    5. Cell Filtrates
    6. Microorganisms (BSL2)
    7. Other Biological Samples

*If your sample type is not listed above please let us know and we can discuss other ways of analyzing your sample!

GC-MS Picture                          FA Equipment
                           HP 6890/5973 GC/MS                                                                     Equipment for Fatty Acid Extraction

Classes of Fatty Acids Monitored:

FA

    1. C12:0 Lauric (Dodecanoic) Acid
    2. C14:0 Myristic (Tetradecanoic) Acid
    3. C15:0 Pentadecanoic Acid
    4. C16:0 Palmitic (Hexadecanoic) Acid
    5. C17:0 Margaric (Heptadecanoic) Acid
    6. C18:0 Stearic (Octadecanoic) Acid
    7. C18:1 Oleic Acid
    8. C18:2 Linoleic Acid
    9. C18:3 Linolenic Acid
    10. C20:0 Eicosanoic (Arachidic) Acid
    11. C20:3 Dihomo- gamma linoleic Acid
    12. C20:4 Arachidonic Acid
    13. C22:0 Behenic (Docosanoic) Acid

 

How We Analyze Your Samples:

FA Standards are first added to samples. Lipids are then extracted (saponification is done for total FA only). Chemical derivitization occurs (methylation). And the sample is injected into the GC/MS for analysis. Finally, data is collected and organized into a report.
 

How the Results Are Reported:

For Free Fatty Acids Analysis:GC Profile

    1. Classes of the free fatty acids in the sample
    2. Concentration of each free fatty acid (µg/ml)
    3. Percentage (%) of each fatty acid out of the total amount of fatty acids

For Total Fatty Acids Analysis:

    1. Classes of the total fatty acids in the sample
    2. Concentration of each fatty acid (µg/ml)
    3. Percentage (%) of each fatty acid out of the total amount of fatty acids

 

Pricing: TBD (Project Dependent)

1Project is also a collaboration between the AIC and the Metabolomics Service Center

 

2) Sugars or Monosaccharides Analysis with GC/MS

In addition to fatty acid analysis, the AIC is also offering monosaccharide analysis using our HP 6890/5973 GC/MS.
Currently, we are able to analyze the following sample types:

    1. Plasma
    2. Blood
    3. Cell lines
    4. Tissues
    5. Cell Filtrates
    6. Microorganisms (BSL2)
    7. Other Biological Samples

 

How We Analyze Your Samples:

First, internal standards will be added to your samples before the actual sample extraction takes place. Next, the sugars are hydrolyzed through acid-catalyzed reactions. The sugars are then reduced, chemically derivatized and then injected into the GC/MS for analysis. Finally, the data is collected and organized into a report for the user.
 

Classes of Sugars Monitored:

Sugars

    1. Pentoses
    2. Hexoses
    3. Aminosugars
    4. Glucose
    5. Galactose
    6. Mannose
    7. Fructose
    8. Rhamnose
    9. Ribose
    10. Arabinose
    11. Xylose
    12. GlcN
    13. Others*

 

*Please inquire about other sugars that are not on this list to see if we are able to analyze them

 How the Results Are Reported:

    1. Classes of the monosaccharides in the sample
    2. Concentration of each sugar/monosaccharide (µg/ml)
    3. Percentage ratio of each sugar out of the total sugars (%)

 

Pricing: TBD (Project Dependent)

 

3) Other Services for Biochemical and Physiological Analysis

The Analytical Instrumentation Core possesses multiple analytical instruments to fit the ongoing needs of a wide variety of users. AIC personnel are able to perform various analyses that cater to the needs of individual projects. The analytical instruments include: GC, GC/MS, HPLC machines, FPLC machine, Electrophoresis, Spectrophotometers (plate readers), Blood Analyzer, Chemistry Analyzer, Seahorse Oxygen (pH) Analyzer, qPCR (2 StepOne Pluses) and qRT-PCR (ABI 7900) and instruments for the multiplexing of proteins and cytokines. Our services are most beneficial for: starting projects, laboratories that are lacking in personnel and expertise, epidemiological studies and clinical research. As always, all charges will be reasonable, negotiable and will depend on the specific needs of the project.

 

Contact

For further information on our Analytical Services or to schedule a meeting to develop a project, please contact:

Matthew Au
AIC Lab Manager
670 Albany St Rm 232
Boston MA 02118
mau115@bu.edu

617-414-2964
Lynn Deng
Director of Analytical Instrumentation Core
670 Albany St Rm 232
Boston MA 02118
lynndeng@bu.edu

617-638-6920